Since the main energy of soil microorganisms is from sucrose and free simple sugars that should be hydrolyzed and broken down with the enzyme invertase (S-SC) (FRANKENBERGER & JOHANSON, 1983), invertase is more efficient than other enzymes to reflect soil fertility and biological activity.

Reaction medium for acidic invertase assay (vacuole and cell wall) was composed by 200 [micro]L of sodium acetate buffer 1.0 M pH 4.7, 100 [micro]L of Mg[Cl.sub.2] 0.1 M and 400 [micro]L of sucrose 1.0 M, with added water up to 2.000 [micro]L.

Clear supernatant were used for the estimation of glucose, trehalose, trehalase, amylase and invertase. Glucose and trehalose contents were estimated according to Hartel et al.

Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays.

This biochemical process consists of the incomplete oxidation of sugar, sucrose, which undergoes hydrolysis promoted by the action of invertase enzyme resulting in glucose and fructose.

Hudson, "The analysis of gamma-methylfructoside mixtures by means of invertase. I," Journal of the American Chemical Society, vol.

Karabournioti and P Zervalaki, "The effect of heating on honey HMF and invertase," Apiacta, vol.

Before they were studied, they were characterized by the determination of their moisture content, electrical conductivity, specific rotation, and invertase activity as well as by using classical visual and organoleptic testing.

The glucosamine content, the scores of molds and yeasts and the activity of the invertase enzyme were used as indicatives of conservatives in action in the masses pizzas, as well as moisture, pH and acidity were carried out to estimate physicochemical characteristics (Instituto Adolfo Lutz, 1985).

The reason for increasing the reducing sugar might be due to the presence of invertase enzymes but invertase enzymes works properly at 4.6 pH and 50[degrees]C temperature and since the temperature was ambient in this condition, thus making it inadequate for activity of invertase enzyme.

Soluble invertase expression is an early target of drought stress during the critical, abortion-sensitive phase of young ovary development in maize.

Other author revealed that the conversion of sucrose to glucose and fructose and also organic acids and alcohol in palm sugars were under the invertase reaction [15].

He also takes an enzyme blend of amylase, cellulase, lactase, glucoamylase, alpha-galactosidase and invertase.

Kinetics of sucrose conversion to fructooligosaccharides using enzyme (invertase) under free condition.

[12] The main enzymes in honey are invertase, amylase and glucose oxidase.