Explant Culture Technique Leaf internode and root explants excised from 21-day-old in vitro seedlings grown on MS basal medium were cut into 0.5-1.0 cm pieces and placed horizontally in culture tubes containing 20 mL MS basal salt medium supplemented with 24-D (4.52 9.04 13.56 and 18.04 M) or TIBA (1.0 2.0 4.0 and 6.0 M) or NAA (2.67 5.35 10.7 and 16.05 M) with BAP (2.22 M) or IBA (2.45 4.90 9.80 and 14.70 M) with BAP (2.22 M).

1A); eyebridge of three rows of facets, separated by one-facet diameter width; interocular suture present, obtuse; ocular bristles absent; frontal scar patch crown-shaped with median band of alveoli reaching interocular suture; labellum bulbous, setose; only first palp segment present on specimen, 71 pm long; scape cylindrical, pedicel spherical, 0.8 x length of scape; flagellomeres elongate, symmetrically nodiform; first flagellomere with node very elongate, more than three times as long as internode; other flagellomeres with internode as long as node; each flagellomere with two ascoid attachment points, ascoids missing from specimen; some flagellomeres with spiniform sensillae.

The total number of internodes and the number of internodes per stalk bored were also recorded.

The leaf sheath's function as stiffening structure has been lost or is only minimal in the basal internodes (leaf sheaths occasionally are dried and fall off), while it increases towards the apical internode.

To evaluate anatomical structure and monitor the lignification process, cross-sections of the young stem were performed, from 1st to 8th internodes and of adventitious roots (2-17.5 cm from the root cap) of individuals aged between 9 and 20 months.

Leaf, internode, cotyledon and were excised under aseptic conditions in laminar air flow cabinet.

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Table 5: Mean comparison for Middle internode length.

The data for different parameters such as cane thickness, number of internodes per plant, cane height, millable canes, cane yield were recorded at harvest on plot basis and then converted into hectares.

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At the beginning of spring (late September), the vegetative apex differentiates into reproductive, internode elongation occurs, and by early October plants can be in the boot stage.

The two organizations have conducted research using the eui (elongated uppermost internode) mutant of rice, which has a longer internode than conventional rice.

A change in the plant height and internode length was observed compared with control plants.